{"id":2679,"date":"2004-03-01T11:08:03","date_gmt":"2004-03-01T10:08:03","guid":{"rendered":"http:\/\/www.isidorolab.com\/public\/?p=2679"},"modified":"2021-09-05T11:13:19","modified_gmt":"2021-09-05T09:13:19","slug":"preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes","status":"publish","type":"post","link":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/","title":{"rendered":"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2679\" class=\"elementor elementor-2679\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-79422a14 elementor-section-stretched elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"79422a14\" data-element_type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6a0fe900\" data-id=\"6a0fe900\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-653868ca elementor-widget elementor-widget-text-editor\" data-id=\"653868ca\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Carini+R&amp;cauthor_id=14970255\">Rita Carini<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/14970255\/#affiliation-1\"> 1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Castino+R&amp;cauthor_id=14970255\">Roberta Castino<\/a>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=De+Cesaris+MG&amp;cauthor_id=14970255\">Maria Grazia De Cesaris<\/a>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Splendore+R&amp;cauthor_id=14970255\">Roberta Splendore<\/a>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=D%C3%A9moz+M&amp;cauthor_id=14970255\">Marina D\u00e9moz<\/a>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Albano+E&amp;cauthor_id=14970255\">Emanuele Albano<\/a>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Isidoro+C&amp;cauthor_id=14970255\">Ciro Isidoro<\/a><\/p>\n<p>\u00a0<\/p>\n\n<h3 class=\"wp-block-heading\">Affiliation<\/h3>\n\n<p><sup>1<\/sup> Laboratory of Pathology, Dipartimento di Scienze Mediche, Universit\u00e0 del Piemonte Orientale A. Avogadro, via Solaroli 17, 28100 Novara, Italy.<\/p>\n\n<p>PMID: <strong>14970255<\/strong><\/p>\n<p>DOI: <a href=\"https:\/\/doi.org\/10.1242\/jcs.00923\" target=\"_blank\" rel=\"noreferrer noopener\">10.1242\/jcs.00923<\/a><\/p>\n\n<p>J Cell Sci. 2004 Mar 1;117(Pt 7):1065-77. doi: 10.1242\/jcs.00923. Epub 2004 Feb 17.<\/p>\n<p>\u00a0<\/p>\n<hr \/>\n<h2 class=\"wp-block-heading\">Abstract<\/h2>\n\n<p>A short period of hypoxia reduces the cytotoxicity produced by a subsequent prolonged hypoxia in isolated hepatocytes. This phenomenon, termed hypoxic preconditioning, is mediated by the activation of adenosine A2A-receptor and is associated with the attenuation of cellular acidosis and Na+ overload normally occurring during hypoxia. Bafilomycin, an inhibitor of the vacuolar H+\/ATPase, reverts the latter effects and abrogates the preconditioning-induced cytoprotection. Here we provide evidence that the acquisition of preconditioning-induced cytoprotection requires the fusion with plasma membrane and exocytosis of endosomal-lysosomal organelles. Poisons of the vesicular traffic, such as wortmannin and 3-methyladenine, which inhibit phosphatydilinositol 3-kinase, or cytochalasin D, which disassembles the actin cytoskeleton, prevented lysosome exocytosis and also abolished the preconditioning-associated protection from acidosis and necrosis provoked by hypoxia. Preconditioning was associated with the phosphatydilinositol 3-kinase-dependent increase of cytosolic [Ca2+]. Chelation of free cytosolic Ca2+ in preconditioned cells prevented lysosome exocytosis and the acquisition of cytoprotection. We conclude that lysosome-plasma membrane fusion is the mechanism through which hypoxic preconditioning allows hepatocytes to preserve the intracellular pH and survive hypoxic stress. This process is under the control of phosphatydilinositol 3-kinase and requires the integrity of the cytoskeleton and the rise of intracellular free calcium ions.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t<div style='text-align:center' class='yasr-auto-insert-visitor'><!--Yasr Visitor Votes Shortcode--><div id='yasr_visitor_votes_5677efe9e904e' class='yasr-visitor-votes'><div class=\"yasr-custom-text-vv-before yasr-custom-text-vv-before-2679\">Clicca per votare questo articolo!<\/div><div id='yasr-vv-second-row-container-5677efe9e904e'\r\n                                        class='yasr-vv-second-row-container'><div id='yasr-visitor-votes-rater-5677efe9e904e'\r\n                                      class='yasr-rater-stars-vv'\r\n                                      data-rater-postid='2679'\r\n                                      data-rating='0'\r\n                                      data-rater-starsize='32'\r\n                                      data-rater-readonly='false'\r\n                                      data-rater-nonce='e156304cd3'\r\n                                      data-issingular='false'\r\n                                    ><\/div><div class=\"yasr-vv-stats-text-container\" id=\"yasr-vv-stats-text-container-5677efe9e904e\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" width=\"20\" height=\"20\"\r\n                                   class=\"yasr-dashicons-visitor-stats\"\r\n                                   data-postid=\"2679\"\r\n                                   id=\"yasr-stats-dashicon-5677efe9e904e\">\r\n                                   <path d=\"M18 18v-16h-4v16h4zM12 18v-11h-4v11h4zM6 18v-8h-4v8h4z\"><\/path>\r\n                               <\/svg><span id=\"yasr-vv-text-container-5677efe9e904e\" class=\"yasr-vv-text-container\">[Total: <span id=\"yasr-vv-votes-number-container-5677efe9e904e\">0<\/span>  Average: <span id=\"yasr-vv-average-container-5677efe9e904e\">0<\/span>]<\/span><\/div><div id='yasr-vv-loader-5677efe9e904e' class='yasr-vv-container-loader'><\/div><\/div><div id='yasr-vv-bottom-container-5677efe9e904e' class='yasr-vv-bottom-container'><\/div><\/div><!--End Yasr Visitor Votes Shortcode--><\/div>","protected":false},"excerpt":{"rendered":"<p>Rita Carini\u00a0 1,\u00a0Roberta Castino,\u00a0Maria Grazia De Cesaris,\u00a0Roberta Splendore,\u00a0Marina D\u00e9moz,\u00a0Emanuele Albano,\u00a0Ciro Isidoro \u00a0 Affiliation 1 Laboratory of Pathology, Dipartimento di Scienze Mediche, Universit\u00e0 del Piemonte Orientale A. Avogadro, via Solaroli 17, 28100 Novara, Italy. PMID: 14970255 DOI: 10.1242\/jcs.00923 J Cell Sci. 2004 Mar 1;117(Pt 7):1065-77. doi: 10.1242\/jcs.00923. Epub 2004 Feb 17. \u00a0 Abstract A short period&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"yasr_overall_rating":0,"yasr_post_is_review":"","yasr_auto_insert_disabled":"","yasr_review_type":"","footnotes":""},"categories":[136],"tags":[55],"class_list":["post-2679","post","type-post","status-publish","format-standard","hentry","category-paper","tag-cancer"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\r\n<title>Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes - IsidoroLab<\/title>\r\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\r\n<link rel=\"canonical\" href=\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\" \/>\r\n<meta property=\"og:locale\" content=\"it_IT\" \/>\r\n<meta property=\"og:type\" content=\"article\" \/>\r\n<meta property=\"og:title\" content=\"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes - IsidoroLab\" \/>\r\n<meta property=\"og:description\" content=\"Rita Carini\u00a0 1,\u00a0Roberta Castino,\u00a0Maria Grazia De Cesaris,\u00a0Roberta Splendore,\u00a0Marina D\u00e9moz,\u00a0Emanuele Albano,\u00a0Ciro Isidoro \u00a0 Affiliation 1 Laboratory of Pathology, Dipartimento di Scienze Mediche, Universit\u00e0 del Piemonte Orientale A. Avogadro, via Solaroli 17, 28100 Novara, Italy. PMID: 14970255 DOI: 10.1242\/jcs.00923 J Cell Sci. 2004 Mar 1;117(Pt 7):1065-77. doi: 10.1242\/jcs.00923. Epub 2004 Feb 17. \u00a0 Abstract A short period&hellip;\" \/>\r\n<meta property=\"og:url\" content=\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\" \/>\r\n<meta property=\"og:site_name\" content=\"IsidoroLab\" \/>\r\n<meta property=\"article:published_time\" content=\"2004-03-01T10:08:03+00:00\" \/>\r\n<meta property=\"article:modified_time\" content=\"2021-09-05T09:13:19+00:00\" \/>\r\n<meta name=\"author\" content=\"ciro_isidoro\" \/>\r\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\r\n<meta name=\"twitter:label1\" content=\"Scritto da\" \/>\n\t<meta name=\"twitter:data1\" content=\"ciro_isidoro\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minuti\" \/>\r\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/#article\",\"isPartOf\":{\"@id\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\"},\"author\":{\"name\":\"ciro_isidoro\",\"@id\":\"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/09655d5679e249988585d483f7fccb44\"},\"headline\":\"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes\",\"datePublished\":\"2004-03-01T10:08:03+00:00\",\"dateModified\":\"2021-09-05T09:13:19+00:00\",\"mainEntityOfPage\":{\"@id\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\"},\"wordCount\":257,\"keywords\":[\"cancer\"],\"articleSection\":[\"Paper\"],\"inLanguage\":\"it-IT\"},{\"@type\":\"WebPage\",\"@id\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\",\"url\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\",\"name\":\"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes - IsidoroLab\",\"isPartOf\":{\"@id\":\"https:\/\/www.isidorolab.com\/public\/#website\"},\"datePublished\":\"2004-03-01T10:08:03+00:00\",\"dateModified\":\"2021-09-05T09:13:19+00:00\",\"author\":{\"@id\":\"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/09655d5679e249988585d483f7fccb44\"},\"breadcrumb\":{\"@id\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/#breadcrumb\"},\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.isidorolab.com\/public\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.isidorolab.com\/public\/#website\",\"url\":\"https:\/\/www.isidorolab.com\/public\/\",\"name\":\"IsidoroLab\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.isidorolab.com\/public\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"it-IT\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/09655d5679e249988585d483f7fccb44\",\"name\":\"ciro_isidoro\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/bb38c41275320421f71f7cbd289a8451?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/bb38c41275320421f71f7cbd289a8451?s=96&d=mm&r=g\",\"caption\":\"ciro_isidoro\"},\"url\":\"http:\/\/www.isidorolab.com\/public\/index.php\/author\/ciro_isidoro\/\"},false]}<\/script>\r\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes - IsidoroLab","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/","og_locale":"it_IT","og_type":"article","og_title":"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes - IsidoroLab","og_description":"Rita Carini\u00a0 1,\u00a0Roberta Castino,\u00a0Maria Grazia De Cesaris,\u00a0Roberta Splendore,\u00a0Marina D\u00e9moz,\u00a0Emanuele Albano,\u00a0Ciro Isidoro \u00a0 Affiliation 1 Laboratory of Pathology, Dipartimento di Scienze Mediche, Universit\u00e0 del Piemonte Orientale A. Avogadro, via Solaroli 17, 28100 Novara, Italy. PMID: 14970255 DOI: 10.1242\/jcs.00923 J Cell Sci. 2004 Mar 1;117(Pt 7):1065-77. doi: 10.1242\/jcs.00923. Epub 2004 Feb 17. \u00a0 Abstract A short period&hellip;","og_url":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/","og_site_name":"IsidoroLab","article_published_time":"2004-03-01T10:08:03+00:00","article_modified_time":"2021-09-05T09:13:19+00:00","author":"ciro_isidoro","twitter_card":"summary_large_image","twitter_misc":{"Scritto da":"ciro_isidoro","Tempo di lettura stimato":"2 minuti"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/#article","isPartOf":{"@id":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/"},"author":{"name":"ciro_isidoro","@id":"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/09655d5679e249988585d483f7fccb44"},"headline":"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes","datePublished":"2004-03-01T10:08:03+00:00","dateModified":"2021-09-05T09:13:19+00:00","mainEntityOfPage":{"@id":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/"},"wordCount":257,"keywords":["cancer"],"articleSection":["Paper"],"inLanguage":"it-IT"},{"@type":"WebPage","@id":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/","url":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/","name":"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes - IsidoroLab","isPartOf":{"@id":"https:\/\/www.isidorolab.com\/public\/#website"},"datePublished":"2004-03-01T10:08:03+00:00","dateModified":"2021-09-05T09:13:19+00:00","author":{"@id":"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/09655d5679e249988585d483f7fccb44"},"breadcrumb":{"@id":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/#breadcrumb"},"inLanguage":"it-IT","potentialAction":[{"@type":"ReadAction","target":["http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/"]}]},{"@type":"BreadcrumbList","@id":"http:\/\/www.isidorolab.com\/public\/index.php\/2004\/03\/01\/preconditioning-induced-cytoprotection-in-hepatocytes-requires-ca2-dependent-exocytosis-of-lysosomes\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.isidorolab.com\/public\/"},{"@type":"ListItem","position":2,"name":"Preconditioning-induced cytoprotection in hepatocytes requires Ca(2+)-dependent exocytosis of lysosomes"}]},{"@type":"WebSite","@id":"https:\/\/www.isidorolab.com\/public\/#website","url":"https:\/\/www.isidorolab.com\/public\/","name":"IsidoroLab","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.isidorolab.com\/public\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"it-IT"},{"@type":"Person","@id":"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/09655d5679e249988585d483f7fccb44","name":"ciro_isidoro","image":{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/www.isidorolab.com\/public\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/bb38c41275320421f71f7cbd289a8451?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/bb38c41275320421f71f7cbd289a8451?s=96&d=mm&r=g","caption":"ciro_isidoro"},"url":"http:\/\/www.isidorolab.com\/public\/index.php\/author\/ciro_isidoro\/"},false]}},"yasr_visitor_votes":{"stars_attributes":{"read_only":false,"span_bottom":false},"number_of_votes":0,"sum_votes":0},"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/posts\/2679","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/comments?post=2679"}],"version-history":[{"count":3,"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/posts\/2679\/revisions"}],"predecessor-version":[{"id":2682,"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/posts\/2679\/revisions\/2682"}],"wp:attachment":[{"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/media?parent=2679"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/categories?post=2679"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.isidorolab.com\/public\/index.php\/wp-json\/wp\/v2\/tags?post=2679"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}